Mouse anti-RAD51 14B4 antibody (56214) was used in IP assay (immunoprecipitation) using HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40).
Rabbit anti-RAD51 antibody was used for subsequent WB detection of immunoprecipated RAD51.
Whole cell extract was used as input.

RAD51 antibody 14B4 (56214) detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
RAD51 antibody 14B4 (562148) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (56214) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (56214) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.